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Key Features |
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Precision
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Reproducibility |
Repeatability |
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To know the fertility potential of male, it is must to perform ‘Semen Analysis’. Semen is body fluid, viscous in nature, it contain Sperms & Seminal plasma. During evaluation of semen following points should be given importance :
- Abstinence period
- Method of semen collection & collection place
- No dilution of semen
- Bring all the sperm in single layer.
- Define micrometric area.
- Avoid effect of air current.
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No Dilution Of Semen ▼ |
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Preferably semen analysis is to be done without adding any diluents.
Sperm Meter is designed to eliminate the requirement of semen dilution. |
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Dimensional Constancy ▼ |
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Owing of viscous nature of semen it forms a smear of distinct thickness. Hence during microscopic analysis dimensional constancy of smear thickness is an essential requirement Sperm Meter is design to achieve 10 micron thick smear.
This has following advantages :-
- Avoids overlapping of sperms.
- Allows free head movements of sperms in all directions.
- Facilitates the serial microphotography in computational image processing.
- Quantitative and qualitative assessment of sperm motility in term of sperm concentration within the same focus of microscope.
- Permits easy calculations.
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Defined Micrometric Area ▼ |
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Determination of precise sperm concentration and motility (quantitative & qualitative) requires well defined micrometric area.
Sperm Meter is designed to give 100 squares of 0.100 mm x 0.100 mm in the form of surface graticule of 1 mm thick cover slip.
Dimensional Consistency & micrometric area are Accredited by certified authority. |
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Significance of Surface Gaticule ▼ |
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Microscopic examination is based on constant depth of focus. In SpermMeter the surface graticule has been created on inner surface of the cover slip which facilitates the view of sperm and graticule in same focus of microscope without disturbing the dimensional constancy.
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| Sperm concentration |
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No. of Sperm in 10 square x depth x dillution |
x 104/ml |
area counted |
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No. of Sperm x 10 x 1 |
x 104/ml |
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0.1 |
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No. of Sperm x 106/ml |
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Qualitative And Quantitative Assessment of Sperm Motility ▼
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The prime factor in semen analysis’s is assessment of sperm motility (quantitative and qualitative) with reference to sperm concentration within ht same focus of microscope.
During transition of objective optics to higher magnification it will PERTURB the sample by producing air currents, which will affect the visual field. Hence during assessment of Sperm motility undisturbed visual field is the prime requirement.
Remarks : During microscopic examination, handling with 40x objective perturbs semen sample by creating more air currents since distance between object and 40x objective lens is only 0.560 mm.(approx).
Hence semen sample needs to be assessed using 10x or 20x objectives only. |
Sperm Meter has been designed to avoid use of 40x objective lens by providing :
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Elevated edge restricts 40 x objective lens to align in focused position.
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1 mm thick cover slip with inner surface graticule is used hence focusing of sperm and graticule within the same focus of microscope with 40 x objective lens is not feasible.
| The use of Sperm Meter avoids air currents as cover slip is provided with metallic ring which acts as an air lock system. |
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