Place :

Sperm Processor Pvt. Ltd.
6, Welcome Nagar, Near Aditya Nagar,
Garkheda Area,
Aurangabad - 431005.

Duration :

5 working days

Schedule :

 1. Acquaintance with Anatomy, Physiology :-

• Sperm
• Ovum
• Embryo

2. Acquaintance with Instruments :-

    Principals, Main Features, Working Pattern :

Imaging System  :-

• Upright microscope
• Inverted microscope
• Stereo zoom microscope

CO₂ Incubator

Ovum Aspiration Pump

Control Temperature System :-

• Microscope Stage Warmer
• Test tube warmer, Petridish Warmer
• Semen Analysis Chamber
• Centrifuge Machine
• Microdrop Pipettes
• Steri Pipette
• Denudation Pipette

3. Acquaintance with Plasticware & Disposable :-

• Medical plastic
• Importance & method of sterilization
• Pyrogen, Rnase & Dnase free

4. Acquaintance with Media :-

• Constituent of Media
• Buffer System
• pH, Osmalarity

5. Acquaintance with Basic Technique :-

General :-

• Preparation of media in CO₂Incubator.
• Preparation of culture media in CO₂ Incubator.
• Preparation of Droplet.

Ovum :-

• Preparation & handling in the microscope.
• Denudation of OCC by mechanical method.
• Denudation of OCC by enzymatic method.

Sperm :-

• Aspiration, Layering, Mixing

Embryo :-

• Change of Media

6. Acquaintance with Procedure :-

Ovum OCC :-

• Identification & Grading of OCC.
• Handling of OCC in Microdrop
• Denudation of OCC

7.  Semen :-

• Calculation of total functional motile sperm.
• Swim-up & Swim-down procedure.

8. Sperm Ovum Interaction :-

• Semen Insemination.

9. Embryo :-

• Pronudeus stage :  Zygote score
• Cleavage stage    :  Blastomere detail & Embryo                              grading
• Blastocyst          :  Grading of Blastocyst